Table 2.
Control |
RA |
Citral |
||||
---|---|---|---|---|---|---|
Proximal | Distal | Proximal | Distal | Proximal | Distal | |
Stereocilia number (mean±s.e.m.) | 79.3 ±5.6 | 48.6 ±3.2 | 54.4 ±3.1 | 26.0 ±1.8 | 104.6 ± 10.1 | 56.9 ±3.2 |
nBPs = 8 | nBPs = 8 | nBPs = 6 | nBPs = 6 | nBPs = 8 | nBPs = 8 | |
nHCS = 96 | nHCS = 65 | nHCS = 54 | nHCS = 53 | nHCS = 68 | nHCS = 76 | |
Hair bundle height (µm) | 2.14 ±0.23 | 3.03 ±0.13 | 3.48 ±0.17 | 2.79 ±0.23 | 1.42 ±0.12 | 1.80 ±0.21 |
nBPs = 8 | nBPs = 8 | nBPs = 9 | nBPs = 9 | nBPs = 6 | nBPs = 6 | |
nHCS = 169 | nHCS = 282 | nHCS = 218 | nHCS = 181 | nHCS = 156 | nHCS = 114 | |
Luminal surface area (µm2) | 28.4 ±2.44 | 11.17 ±0.85 | 14.82 ±1.48 | 9.15 ±0.72 | 33.07 ±3.37 | 13.74 ±0.94 |
nBPs = 15 | nBPs = 15 | nBPs = 20 | nBPs = 20 | nBPs = 12 | nBPs = 12 | |
nHCS = 242 | nHCS = 228 | nHCS = 359 | nHCS = 322 | nHCS = 189 | nHCS = 197 |
Quantitative analyses of the hair cell phenotypes that formed in proximal and distal regions of E6.5 BPs treated with control media or media supplemented with RA or citral. Values shown are mean±s.e.m.