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. 2015 Jan 5;112(3):E287–E296. doi: 10.1073/pnas.1422852112

Fig. 2.

Fig. 2.

lit-1/Nlk promote seam cell division asymmetry and oppose differentiation. (A) lit-1(RNAi) (Right) causes decrease in seam cell number, assayed in young adults (YA) using scmp::gfp, compared with empty vector RNAi controls (Left). (B) Animals doubly marked with scmp::gfp and ajm-1::gfp were used to monitor fusion (differentiation) of seam cells to syncytium during the L2 developmental stage. In the WT, four sisters for each V seam cell lineage are evident just after the L2 asymmetric divisions. The posterior sisters remain seam cells, whereas the anterior sisters differentiate by fusing to the syncytium. (C) lit-1(RNAi) caused premature fusion (differentiation) of seam cells (yellow D) in L2 stage asymmetric division. S, seam cell; D, differentiated cell. (D) lit-1(RNAi) seam cell number phenotype is enhanced in combination with the lit-1(ne1991) hypomorphic mutation. (E) Schematic representation of seam cell division pattern in WT, lit-1(RNAi) and lit-1(RNAi);lit-1(n1991) animals. Seam cell fate: green circles; differentiation fate: red squares. Dotted line, variable fates (seam or differentiation). Anterior is to the left. Error bars indicate ±SEM. *P < 0.05, **P < 0.01, ***P < 0.005, two-tailed t test. (Scale bar, 50 μm.)