Fig. 1.

Single cell-derived clonal cultures from primary GBMs show a neural precursor phenotype and are tumorigenic. (A) Functional characterization of single cell-derived clones. Glioblastoma samples were dissociated into single cell suspensions. Cells were then sorted using stem cell markers to enrich for clonogenic activity. Single live cells were then expanded in EGF/FGF media and analyzed functionally and genomically. (B) Immunostaining of a representative xenograft shows tumor formation with an infiltrative phenotype reminiscent of human GBM cell behavior in situ (green, human nestinl; red, human GFAP; blue, DAPI).