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Midge life cycle and AFP purification by ice affinity purification. (A) Schematic of the 1-y midge life cycle. (B) Adult. (C) A beaker containing 50 g of frozen midges. (D) The soluble fraction obtained by centrifugation of the insect homogenate. (E) The ice fraction after one round of ice affinity purification. (F) The ice fraction after three rounds of ice affinity purification.
