Fig. 4.

Replication of RV-1BM at 37 °C is partially restored in airway cells deficient in RLR or type I IFN receptor signaling. Primary airway cells derived from WT, Mavs^−/−, or Ifnar1^−/− mice were inoculated with RV-1BM, MOI 0.01, at 33 °C for 1 h, and then incubated for 24, 48, or 72 h at 33 °C or 37 °C. Cell lysates were prepared at the indicated time points and titers were determined by plaque assay on HeLa cells. Asterisks indicate a significant difference in the titer observed in WT vs. KO airway cells incubated at the same temperature (P < 0.05).