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. 2015 Jan 5;112(3):911–916. doi: 10.1073/pnas.1423603112

Fig. 2.

Fig. 2.

DRM3 acts downstream of the production of siRNAs. (A) Northern blots show accumulation levels of small RNAs at 5S rDNA, AtSN1, and siRNA1003 loci. The siRNA02 and miR165a serve as two internal controls. (B) Average distribution of 24-nt siRNA reads over drm2 CHH hypo-DMRs. The siRNA abundance is significantly reduced compared with WT in all mutants (P < 2.2e−16; Wilcoxon signed rank test). Levels of 24-nt siRNAs in WT and mutants over previously defined siRNA clusters affected in the “downstream” (C) and “upstream” (D) RdDM mutants. The 24-nt siRNA levels are only significantly reduced in drm3 (P = 4.611e−10; Wilcoxon signed rank test), drm2 (P < 2.2e−16; Wilcoxon signed rank test), and nrpe1 (P < 2.2e−16; Wilcoxon signed rank test) compared with those of WT in the downstream clusters (C).