Fig. 4.
Prenodes express different Nav isoforms. (A–C) Hippocampal neuron cultures at (A) 14, (B) 17, or (C) 24 DIV immunostained for Nav1.1, Nav1.2, Nav1.6, AnkG, GAD, and/or PLP as indicated, illustrating the different Nav α-subunit isoform expression in prenodes at different time points. *Neuronal cell body. (Scale bar: 25 µm.) (D) Percentage of axons with clusters of AnkG, which are colocalized with Nav1.1, Nav1.2, or Nav1.6 at different DIVs. Quantifications were performed in mixed cultures and cultures of purified neurons with OCM. The means ± SDs of three independent experiments are shown. For each experiment, at least 100 neurons were analyzed.