Fig. 6.

C-terminal Hypervariable region regulates Rac1 recruitment. MCF-7 cells overexpressing Eos-labeled wild type and mutants of C-tail of Rac1 were imaged during spreading on collagen. (A−E) Bright field, 2D recruitment, and 2D on-rate maps show the membrane recruitment of C-tail (A), PPP > AAA (B), ΔPPP (C), RKR > AAA (D), and pm-Eos (E) molecule. Scale bar is 5 μm. (F) Sequences and the corresponding mutations of the C-terminal HV region of Rac1. (G) Representative graph shows the coefficient of variance for each construct, obtained from gray values of 2D on-rate maps. Error bars represent SEM for cells. *P < 0.05. Values for each construct represent data pooled from several independent experiments C-tail (6 cells, n = 3.6 × 10⁴ trajectories), PPP > AAA (6 cells, 3.7 × 10⁴ trajectories), ΔPPP (5 cells, 2.9 × 10⁴ trajectories), RKR > AAA (5 cells, 3.0 × 10⁴ trajectories), and pm-Eos (6 cells, 2.8 × 10⁴ trajectories).