Fig. 2.

Ab-dependent cell-mediated cytotoxicity and receptor degradation induced by anti-HER3 Abs. (A and B) N87 cells were treated for 3 h with the indicated mAbs. Protein samples were subjected to immunoblotting by using the indicated Abs, and signals were quantified. (C) The experiment shown in A was performed on six other cancer cell lines, except that GAPDH was used as a loading control. (D) Luciferase-expressing BXPC3 cells were incubated with the indicated mAbs and secondarily with human peripheral blood mononuclear cells (for 24 h). Cell killing was detected by measuring luminescence after the addition of luciferine. ***P < 0.001 (ANOVA and post hoc tests).