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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Methods Enzymol. 2014;547:251–273. doi: 10.1016/B978-0-12-801415-8.00014-X

Figure 14.5.

Figure 14.5

TPM of H2O2 presence in cytoplasm and mitochondria of HT22 cells (A) PF6-AM (green; white in the print version) staining peroxide in the cytoplasm of HT22 cells with exogenously applied 50 μM H2O2 for 3 min (left panel) and 38 min (right panel). Hoechst 33342 (blue; light gray in the print version) shows nuclear staining of live cells. (B) MitoPY1 (green; white in the print version) staining of H2O2 in mitochondria. Here, cells were either untreated (left panel) or treated with 50 nM rotenone, a chemical which interferes with the mitochondrial electron transport chain and produces ROS. Figure modified from Guo et al. (2013).