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. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Methods Enzymol. 2014;547:251–273. doi: 10.1016/B978-0-12-801415-8.00014-X

Figure 14.6.

Figure 14.6

TPM of cytoplasmic H2O2 stained with PF6-AM in astrocytes. Astrocytes are incubated with 2 mM D-ala +FAD for (A) 1 min, (B) 6 min, and (C) 25 min before staining with PF6-AM (green; gray in the print version) and Hoechst 33342 (blue; white in the print version). Cytoplasmic H2O2 concentration accumulates the longer the D-ala + FAD treatment as shown in greater green intensity after 25 min compared to 1 min. Figure modified from Guo et al. (2013).