FIG 4.

Effect of TbRGG3 silencing on growth. (A) The RNAi vector p2T7-177-TbRGG3 was transfected into the PF strain 29-13 of T. brucei. RNAi was induced by the addition of 2.5 μg/ml tetracycline to the cell medium. Cell growth was monitored in triplicate cultures of uninduced and induced cells for 12 days. (B) qRT-PCR analysis of TbRGG3 mRNA levels (induced/uninduced) on day 3 postinduction. RNA levels were standardized to 18S rRNA, and the value represents the mean and standard error of 6 determinations.