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Effect of TbRGG3 knockdown on mitochondrial RNA levels. RNA was isolated from PF T. brucei on day 3 or 4 postinduction. RNAs were quantified by qRT-PCR using primer sets specific for selected never-edited, pan-edited, and minimally edited RNAs. Relative RNA abundance indicates RNA levels in Tet-induced cells compared to those in uninduced cells. RNA levels were standardized to 18S rRNA levels, and values represent the means and standard errors of 6 to 15 determinations.
