FIG 2.

SNF^r results in alterations in the coding sequence of TGVAND_290860. The T → A SNP in the SNF-resistant progeny results in an early stop codon in the predicted amino acid transporter gene, TGVAND_290860 (red box). VAND and P1_16VB are SNF^s, and VAND-SNF^r and P1_49VBSF are SNF^r. The TGVAND_290860 gene was sequenced from additional SNF-resistant strains and found to have either conservative amino acid residue changes in the GT1-SNF^r and RH-SNF^r lines compared to the wild type (WT) (green boxes) or a large deletion in CTG-SNF^r (blue box).