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Disruption of TGVAND_290860 in RH using CRISPR. SNFr parasites were created using the six CRISPR constructs. Two clones from each resistant pool were sequenced at the respective target site. The sequence from around the guide RNA is shown for the RH wild type and clones from C1 to C6, as indicated. Clones exhibited small indels or large insertions. PAM, protospacer adjacent motif. Numbering refers to the bp position in the TGVAND_290860 CDS.
