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Plaque assay of CRISPR-transfected parasitesa
| sgRNA | No. of SNFr plaques at indicated no. of parasites/well |
% SNFrb | No. of integrants/no. of clones tested | |
|---|---|---|---|---|
| 1 × 104 | 1 × 103 | |||
| sgRNA-C6 | Lysed | 89 | 8.9 | 9/11 |
| sgRNA-C1 | Lysed | 64 | 6.4 | 7/11 |
| sgRNA-UPRT | 0 | 0 | 0 | |
A DHFR* selection cassette was used in all cases.
Calculated as no. of plaques/no. of seeded parasites.
