FIG 2.

Expression of Rvs proteins in C. albicans. The results of immunoblot analysis of C. albicans strains in which the different Rvs proteins were C-terminally tagged with either 3HA (triple hemagglutinin epitope) or 6MYC (sextuple myc epitope) are shown. Cells were grown to mid-exponential phase in YPD medium, and equal amounts of total lysates were separated on a 10% acrylamide gel, except for RVS167-HA, for which five times less total protein was loaded because of the high expression level. Blots were probed with antibodies directed against HA (left) or MYC (right) and against hexokinase (HXK) as a loading control.