FIG 3.

Specific BAR-BAR interactions as revealed by yeast two-hybrid assays. (A) Schematic representation of the RVS167 and RVS167-3 constructs used in the two-hybrid experiment. Constructs harbored the BAR domain either with or without helix 0 (+H0 or −H0). (B) Serial dilutions of strains cotransformed with the indicated bait and prey constructs were spotted on minimal plates with histidine (His⁺), without histidine (His⁻), without histidine and containing 20 mM 3-amino-1,2,4-triazole (His⁻ 20 mM 3AT), and without adenine (Ade⁻). Weak interactors activated only the HIS3 reporter and showed growth on His⁻ plates, while strong interactors activated both HIS3 and ADE2 reporters and showed growth on His⁻/20 mM 3AT and Ade⁻ plates. *, Rvs167-3 showed weak self-activation and growth on His⁻ plates in the presence of an empty prey plasmid (see also Fig. S4 in the supplemental material).