FIG 7.

Rvs161/Rvs167 and Rvs162/Rvs167-3 heterodimers bind membranes in vitro. Coomassie-stained SDS-PAA gels used in lipid cosedimentation experiments are shown. Purified Rvs162(+H₀)/Rvs167-3(−H₀) (A) and Rvs161(−H₀)/Rvs167(−H₀) (B) heterodimers were incubated at 5.3 μM and 1.7 μM, respectively, for 30 min at room temperature with Folch liposomes containing PS (15%) or PS and PI(4,5)P₂ (5%) lipid species and then ultracentrifuged to separate the liposome-bound fraction (P) from the unbound fraction (S). An asterisk (*) indicates the 6His-MBP contaminant in the Rvs167-3/Rvs162 preparation. Similar results were obtained for the Rvs161/Rvs167 heterodimer at a higher protein concentration (see Fig. S6 in the supplemental material).