TABLE 2.
Pairwise testing of BAR interactions among Rvs family members
| Bait | Interaction with preya |
||||
|---|---|---|---|---|---|
| CaRVS161 | CaRVS167 | CaRVS167-3 | CaRVS162 | CaRVS167-2 | |
| CaRVS161 | − | +++ | − | − | − |
| CaRVS167 | +++ | + | − | − | + |
| CaRVS167-3 | − | − | + | +++ | − |
| CaRVS162 | − | − | +++ | − | +++ |
| CaRVS167-2 | − | + | − | +++ | + |
Interactions were assessed by Y2H analysis using a LacZ (β-galactosidase) reporter. −, no interaction (white); +, weak interaction (light blue staining after >8 h of incubation); +++, strong interaction (dark blue staining after ∼30 min of incubation).