TABLE 1.
Relative metabolite levels of MEP pathway intermediates detected by IP-RP-UPLC-MS/MS in control and API-minus late-stage (IV and V) gametocytes
| MEP intermediate | Ratioa in: |
|
|---|---|---|
| Control gametocytesb | API-minus gametocytesc | |
| DOXP | 0.10 ± 0.07 | ND |
| MEP | 0.75 ± 0.21 | ND |
| CDP-ME | ND | ND |
| CDP-MEP | 2.00 ± 1.41 | ND |
| MEcPP | 0.65 ± 0.14 | ND |
| HMBPP | 0.13 ± 0.04 | ND |
| IPP | 2.78 ± 0.18 | 2,733 ± 387 |
a
The analyte-to-internal standard ratio (response) was calculated for each metabolite by dividing the area of the analyte peak by the area of the internal standard (ISPP) peak. Values are means ± SD from two biological replicates. ND, not detected.
b
Control gametocytes without IPP supplementation.
c
API-minus gametocytes with IPP supplementation until were recovered for analysis (13 days after gametocytogenesis was induced).