Fig 4. Neuronal cell body-associated 263KA647 particles are intracellular.
To provide formal proof of the intracellular localization of 263KA647 particles (magenta) in cell bodies, neuronal cultures at 4 dpe were cooled to 4°C to inhibit endocytosis and treated with Alexa Fluor 488-tagged wheat germ agglutinin (green) to specifically label the cell surface. After labeling the cells were rinsed, fixed, and imaged by confocal microscopy. An orthogonal projection of a single optical slice from near the middle of the cell in a merged image is shown.