Fig 9. Fluorescent particles internalized by non-neuronal cells are PrPres.
Non-neuronal cultures were treated with 263KA647 for 2 days and then subjected to PrPres-specific immunostaining with anti-PrP mAb 132. Internalized fluorescent aggregates (A and E) were specifically labeled with mAb 132 only when the samples were treated with GdnSCN (compare B to F). A negative control culture (I-L) treated with GdnSCN but not 263KA647 showed no fluorescence in the Alexa Fluor 647 channel (I) or immunostaining with mAb 132 (J). A merge of the Alexa-PrPres (magenta) and mAb 132 (green) channels is presented with (D, H and L) and without (C, G and K) an overlaid DIC image for each field of view. Merge, white indicates co-localization with equal signal from each channel.