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. 2015 Jan 30;10(1):e0116882. doi: 10.1371/journal.pone.0116882

Fig 2. Jurkat T cells are sensitive to recombinant and native alpha toxin in vitro.

Fig 2

Shown are representative titrations demonstrating the cytotoxicity on Jurkat T cells in vitro of recombinant alpha toxin (A) and native alpha toxin (B) from the supernatants of overnight cultures of S. aureus 8325–4 (circles) or the isogenic (hla-) deletion mutant of 8325–4 (squares). Optical density (OD) increases with viability. The EC50 for recombinant AT = 23.75 ng/ml and for native AT from the supernatant of the 8325–4 strain = 0.03%. The supernatant from S. aureus 8325–4 (hla-) was not cytotoxic (B).