Fig 9. Passive immunization with PND-Ig suppresses bacterial growth in vivo.

Groups of mice (n = 8) were passively immunized with 0.3 mls of PND-Ig or Control Ig 48 hours prior to i.d. challenge with 40 x 10⁶ SAP149, a luminescent derivative of the USA300 stain NRS384 [53]. (A) All mice were photographed at 3 and 6 days after challenge for determination of lesion size as described in Material and Methods. (B) All mice underwent daily in-life imaging for detection of bioluminescence beginning one day after challenge (day 1) as described in Material and Methods. (C) PND-Ig mice were significantly protected from dermonecrosis compared to control-Ig mice at day 3 and day 6 (p = 0.0005, day 3; p = 0.0005, day 6, Mann-Whitney U, one-tailed). (D) PND-Ig mice had significantly less luminescence and bacterial burden at days 5 and 6 after challenge compared to Control-Ig mice (p = 0.046, day 5; p< 0.0001, day 6, Mann-Whitney U, one-tailed).