Fig 11. PND-specific Ab inhibits heptamerization of monomeric AT.

Rabbit RBCs were incubated with AT or with AT pre-incubated with PND-Ig or Control-Ig as described in methods. Following RBC lysis, red cell membranes were pelleted and washed, resuspended in sample reducing buffer and run on 4–15% gradient gels by SDS-PAGE. Excepting the sample in lane 2, samples were not boiled or otherwise heated prior to electrophoresis. AT bands were detected with sheep anti-AT Ab followed by IRDye800-conjugated donkey anti-sheep IgG, and immunoflourescence was detected using an Odyssey imaging system. Image density was quantified using NIH ImageJ software. Results are representative of 3 separate assays.