Fig 12. PND-specific Ab is a minor component of the repertoire of Abs elicited following AT immunization of rabbits and mice.

(A) Two independent lots of commercially available antisera each representing pooled antisera from 2–3 rabbits immunized with full length AT, were evaluated for reactivity with immobilized AT or with peptides by ELISA. The geometric mean titer for antibody reactive with the 1–19 peptide sequence was significantly higher than the titers of Ab reactive with the 119–139 or irrelevant peptide sequences (*p = 0.0002, One way ANOVA; Tukey’s multiple comparison test: *p<0.05, 1–19 vs. 119–139 and 1–19 vs Irrelevant). The titers of Ab reactive with the 119–139 and Irrelevant peptide sequences were not statistically different (B) Individual mouse antiserum obtained from mice immunized with H35L were evaluated for reactivity with immobilized AT or with peptides by ELISA. Geometric mean antibody titers specific for the 1–19 peptide sequence was significantly higher than the responses to either the 119–139 or irrelevant peptide sequences (p = 0.0001, One way ANOVA; Tukey’s multiple comparison test: *p<0.05, 1–19 vs. 119–139 and 1–19 vs Irrelevant). The titers of Ab reactive with the 119–139 and irrelevant peptide sequences were not statistically different. Each immobilized MAP was validated with a control antiserum (not shown). Bars represent geometric means. The lower limit of quantitation for analysis of rabbit and mouse antibody titers were 16 and 4, respectively.