Fig 4. 2-NBDG₆₀ is sensitive to a decrease in blood glucose levels.

A. Representative 2-NBDG kinetic profiles for 3 different fasting groups of mice after injection of a 6 mM (2mg/ml) dose via the tail-vein. There are no significant differences in maximum 2-NBDG-fluorescence between the different fasting groups, confirming that the delivery of 2-NBDG is similar across all animal groups. B. 2-NBDG₆₀ is significantly higher in mice fasted for 6 hours and 12 hours compared with mice that were not fasted (p = 0.02). There are no significant differences between the 6 and 12-hour fasting groups. C. Blood glucose measurements were performed using a Freestyle Lite monitor by drawing 3 μl of blood from the tail of a separate cohort of mice (n = 5). Fasting for 6 hours led to a significant decrease in blood glucose levels. However, fasting for 12 hours did not lead to any further decrease in blood glucose levels. Statistical analysis was conducted using Wilcoxon rank sum tests.