Fig 11. Far and near UV circular dichroism spectra of human plasminogen.

Far UV (185–250 nm) and near UV (250–350 nm) CD spectra were obtained before and after 280 nm light continuous illumination (10 min) of human plasminogen in solution. 280 nm illumination leads to a loss of far UV ellipticity signal at 206 nm (33.4%) and to a red-shift in wavelength of maximum ellipticity (206 nm to 209 nm). There are almost no changes in the near UV CD spectrum of plasminogen with 280 nm illumination. After 10 min of 280 nm ill. the ellipticity signal at the wavelength of maximum ellipticity (285 nm) decreases only by 6%.