Figure 5. Expression of proteins involved in plasmalemmal transport of fatty acids i.e. FAT/CD36 (A), FABPpm (B) and FATP2 (C) in primary rat hepatocytes.

Cells were incubated in high glucose DMEM culture medium without (Control group) or with palmitate (0.75 mM) (Palmitate group) for 16h and 40h. Thereafter primary hepatocytes from each experimental group were prepared as it was described previously in Materials and Methods. Protein concentration was measured by BCA method. Total expression of these proteins were analyzed by western blotting. The data are expressed as the mean ± SEM and are based on six independent determinations (n = 6). *P<0.05 significant difference: Control group vs. Palmitate group. Grey bars are referred as control groups and black bars are referred as palmitate groups.