Table 3. E. coli and B. subtilis strains used in this study.
Strains | Genotype | Reference/Source | |
---|---|---|---|
E. coli | |||
DH5a | F- Φ80lacZM15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rk-,mk+) phoA supE44 thi-1 gyrA96 relA1 λ- | Invitrogen | |
BL21(DE3) | F− ompT gal dcm lon hsdSB(r−B m−B) _ (DE3 [lacI lacUV5-T7gene 1 ind1 sam7 nin5]) | Studier & Moffatt, 1986 [53] | |
B. subtilis | |||
DB002 | trpC2 amyE::(spc Pxyl-SNAP-yuaG) | Donovan & Bramkamp, 2009 [17] | |
IW001 | trpC2 amyE::(spc Pxyl-SNAP) | Donovan & Bramkamp, 2009 [17] | |
BB011 | trpC2 amyE::(spc Pxyl-PHB-GFP) | PHB-pSG1154 —> 168 |