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. 2015 Jan 30;10(1):e0116747. doi: 10.1371/journal.pone.0116747

Figure 7. Association of PI3K/AKT pathway with EMT properties and drug resistance.

Figure 7

(A) MCF-7/ADR-1024 cell viability was measured in the absence or presence of a PI3K inhibitor wortmannin using MTT assay. (B) PI3K inhibitor decreased the expression of genes related to EMT and drug resistance. MCF-7/ADR-1024 cells were incubated with or without 1 μM of wortmanin for 24 hours. mRNA levels of Slug, Snail, Twist and MDR-1 were determined using RT-PCR. (C) P-gp transporter was suppressed by the PI3K inhibitor. MCF-7/ADR-1024 cells were incubated with or without 1 μM of wortmanin for 24 hours. Protein levels were determined by Western blot.