Table 1. Eleven DNA extraction methods and descriptive statistics of the measurements used to evaluate the respective quality of DNA extracted.
| Method * | OD 260/280 | OD 260/230 | C. coli Ct values | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Median | Range | SD | Median | Range | SD | Median | Range | SD | |
| Nucleospin Blood (200 μl WB, SC) | 1.76 | 1.6–1.8 | 0.68 | 1.83 | 1.0–2.1 | 0.30 | 29.65 | 28.8–30.4 | 0.55 |
| Nucleospin Blood L(2 ml WB, SC) | 1.69 | 1.2–1.8 | 0.21 | 1.30 | 1.1–1.9 | 0.53 | 29.64 | 28.7–30.5 | 0.63 |
| Nucleospin Blood XL (10 ml WB, SC) | 1.70 | 1.3–1.8 | 0.12 | 1.35 | 0. 9–1.8 | 0.32 | 29.68 | 28.6–30.6 | 0.62 |
| Nucleospin Blood-Buffy Coat (9 ml BC, SC) | 1.94 | 1.8–2.0 | 0.06 | 2.14 | 1.9–2.2 | 0.11 | 29.68 | 28.7–30.2 | 0.57 |
| Nucleospin Tissue-Buffy Coat (9 ml BC, SC) | 1.80 | 1.7–1.9 | 0.07 | 2.13 | 1.6–2.5 | 0.30 | 29.50 | 28.7–30.2 | 0.60 |
| Modified Blood (9 ml BC, SC) | 1.87 | 1.7–1.9 | 0.05 | 2.30 | 1.9–2.2 | 0.19 | 29.57 | 28.7–30.2 | 0.56 |
| Modified Tissue (9 ml BC, SC) | 1.86 | 1.7–2.0 | 0.07 | 2.13 | 1.6–2.4 | 0.30 | 28.83 | 29.5–30.3 | 0.56 |
| Modified Dx (9 ml BC, SC) | 1.82 | 1.6–1.9 | 0.08 | 2.11 | 1.8–2.3 | 0.15 | 30.20 | 28.4–30.5 | 0.72 |
| Phenol – Chloroform (9ml BC, FC) | 1.67 | 1.5–1.8 | 0.11 | 2.04 | 1.3–2.3 | 0.28 | 30.20 | 26.7–32.7 | 1.39 |
| Charge-Switch (4.5 ml BC, B) | 1.47 | 1.3–1.7 | 0.12 | 0.75 | 0.6–1.2 | 0.14 | 30.12 | 28.7–30.4 | 0.62 |
| In-house (4.5 ml BC, B) | 1.72 | 1.6–1.9 | 0.09 | 1.88 | 1.7–2.1 | 0.13 | 30.07 | 28.8–30.3 | 0.55 |
* Volume, DNA source and technology used are in parentheses; WB = whole blood, BC = buffy coat, SC = silica column, B = beads, FC = Phenol-Chloroform extraction