Figure 6. Protein content of P2X7R in the PCLS of wild type mice and E10.

Immunoblots of PCLS and E10 experiments without (A) and with AMJ2-C11 macrophages (B) after 72 hours incubation. For PCLS the concentration of nano-silica was 1600 µg/ml and for E10 experiments nano-silica concentrations ranging from 50 to 200 µg/cm² flask area and an incubation time of 48 hours were used. Each silica concentration was normalized to the control. γ-Tub is the loading control, n = 6–7.