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. 2014 Oct 15;4(1):112–124. doi: 10.1002/cam4.351

Figure 5.

Figure 5

Overexpression of NDUFB6 suppresses cell proliferation in NDUFB6-downregulated RCC cell lines. (A) Overexpression of NDUFB6 suppresses the growth of RCC cell lines. (a) Protein expression was confirmed by Western blotting with antibodies against NDUFB6 (Sigma-Aldrich), V5 and GAPDH. (b) MTS assay was carried out at 0, 48, 72, and 96 h after transduction of Lv-Control, Lv-NDUFB6, or Lv-LRRC19 into 786-O cells and 769-P cells. The y-axis displays the absorbance at 492 nm (reference wave length 690 nm). The data are expressed as mean ± SEM of quadruplicate determinations, and differences were analyzed by Mann–Whitney U test. Two independent experiments were performed and representative data are shown. *P < 0.05 determined by Mann–Whitney U test. (B) Knockdown of NDUFB6 increases cell proliferation of RCC cell lines. (a) The knockdown of protein expression was confirmed by Western blotting with antibodies against NDUFB6 (Atlas Antibodies AB) and GAPDH. (b) MTS assay was carried out at 0, 72, and 96 h after transfection of Stealth™ RNAi oligonucleotide NDUFB6 siRNA1, NDUFB6 siRNA2, or Stealth™ RNAi Negative Control Duplex into 786-O cells and 769-P cells. The y-axis displays the absorbance at 492 nm (reference wave length 690 nm). The data are expressed as mean ± SEM of quadruplicate determinations, and differences were analyzed by Mann–Whitney U test. Two independent experiments were performed and representative data are shown. *P < 0.05 determined by Mann–Whitney U test.