Skip to main content
. Author manuscript; available in PMC: 2015 Jan 30.
Published in final edited form as: Biochem J. 2015 Jan 1;465(1):103–114. doi: 10.1042/BJ20140813

Figure 4. Impact of the number of viral integration on the toxification potential.

Figure 4

The CYP450 constructs are created as 3'-EGFP fusions with additional IRES-neoR cassette. (A) Shown is the survival of EGFP+ HepG2 cells in percent after two days of incubation with 2.9, 9, 29, 90 and 290 µM 4-IPO. For each construct the mean ± SEM is shown from three experiments. (B) Western blot analysis of transduced HepG2 cells. Detection of the fusion proteins was performed with anti-EGFP antibody. ud: undiluted, −1: virus dilution 10−1; −2 virus dilution 10−2; −3: 10−3 virus dilution; −4: virus dilution 10−4; coop: codon-optimized.