Figure 8. Survival of primary human T-cells expressing the re-engineered P+12 and P+18 CYP4B1 proteins in 4-IPO.

Survival of EGFP+ primary T cells (in %) after 24, 48 and 72 hours of incubation with 2.9, 9, 29, 90 and 290 µM 4-IPO assessed by flow cytometry. For the experiments shown here, co-expression of the CYP4B1 enzymes and EGFP was achieved with the lentiviral vector depicted in Figure 1C. For each construct, the mean ± SEM is shown from at least six experiments. coop: codon-optimized.