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Examples of intrinsic Trp (top row) and extrinsic SYPRO Orange (bottom row) fluorescence thermal melting curves for DNI vaccine formulations after being stored at 4 °C (A), 40 °C (B), and 70°C (C) for 4 weeks. DNI samples were liquid formulation (black), liquid aluminum formulation (red), lyophilized aluminum formulation (blue), liquid aluminum formulation with GLA (purple) and lyophilized aluminum formulation with GLA (green). Lyophilized samples were reconstituted prior to analysis.
