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Total anti-DNI antibody titers (A) and neutralizing antibody titers (B) after one vaccine injection (white circles) and after two vaccine injections (gray circles) for liquid and reconstituted lyophilized Alum (A), Alum+GLA(AG), Alum+DNI (AD) and Alum+GLA+DNI (AGD) vaccines stored at 40 °C for 0–16 weeks. Lyophilized vaccines remain immunogenic even after storage at 40 °C for 16 weeks, where liquid vaccines showed a decrease in immunogenicity after 8 weeks of incubation.
