Table 1.

DNI-containing vaccines incubated at 4, 40 or 70 °C for 0–16 weeks exhibiting fluorescent melting temperatures at 40–50 °C as measured by intrinsic and extrinsic SYPRO Orange methods (●) or only the intrinsic method (○). Sample without detectable transitions are denoted with (○) symbols indicating loss of structural integrity. Lyophilized vaccines maintained DNI thermal melting temperatures during incubation at the three temperatures, where liquid vaccines did not exhibit detectable melting temperatures when stored at temperatures above 4 °C.

		Storage Temperature
Sample	Storage Length (weeks)	4 °C	40 °C	70 °C
Liquid Unbound DNI	0	●
	1	●	●	○
	2	●	●	○
	4	●	●	○
	8	●	○	○
	16	●	○	○
Liquid Alum+DNI	0	●
	1	●	○	○
	2	●	○	○
	4	●	○	○
	8	●	○	○
	16	●	○	○
Liquid Alum+GLA+DNI	0	●
	1	●	○	○
	2	●	○	○
	4	●	○	○
	8	●	○	○
	16	●	○	○
Lyophilized Alum+DNI	0	●
	1	●	●	●
	2	●	●	●
	4	●	●	●
	8	●	●	●
	16	●	●	●
Lyophilized Alum+GLA+DNI	0	●
	1	●	●	●
	2	●	●	●
	4	●	●	●
	8	●	●	●
	16	●	●	●