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. Author manuscript; available in PMC: 2015 Jul 29.
Published in final edited form as: Nature. 2014 Nov 24;517(7536):612–615. doi: 10.1038/nature13967

Figure 4.

Figure 4

Inhibition of Vascular Endothelial Growth Factor Receptor signalling reduces Mycobacterium marinum burden in adult zebrafish

(A) Survival analysis of adult zebrafish infected with 400 CFU (red lines), 4000 CFU (blue lines) or 8000 CFU (green lines) M. marinum. Zebrafish are further grouped into control (dashed lines) or pazopanib treated (solid lines). Log-rank test 400 CFU not significant, 4000 CFU P=0.012, 8000 CFU P=0.029.

(B) Representative image of a necrotic granuloma from a 2 wpi adult Tg(flk1:EGFP) zebrafish infected with M. marinum-cerulean (cyan), and stained for hypoxyprobe (red) and with DAPI (blue). Image is representative of granulomas found in 16 individual animals.

(C) Pooled bacterial burden in pazopanib treated adult zebrafish. Matched T-test.

(D) Comparison of granulomas between control and pazopanib-treated adult zebrafish scored for M. marinum burden as <10 or more than 10 bacteria. Total number of zebrafish analysed = 4 (control), 4 (pazopanib).

Scale bar represents 100 μm. Error bars represent mean ± standard deviation.