Table 1.
Discovery of MneHV-7 and MneHV-6 in saliva of pig-tailed macaques by CODEHOP PCR.
YGD-SLYP pol1,3 | NPFG-PLEN gB2,3 | |||
---|---|---|---|---|
Animal # | 50°C4 | 60°C4 | 50°C4 | 60°C4 |
M02156 | HHV-7 | HHV-6 | HHV-6 | HHV-6 |
M02383 | HHV-7 | nd | nd | nd |
L02393 | HHV-7 | nd | nd | nd |
M03126 | HHV-7 | nd | nd | nd |
M03182 | HHV-7 | nd | nd | nd |
nd not detected
roseolovirus-specific CODEHOPs targeting DNA pol gene;
roseolovirus-specific CODEHOPs targeting glyB gene;
detection of novel roseolovirus sequences was based on acrylamide gel analysis of CODEHOP amplicons;
annealing temperatures used.