Table 4.
MneHV-6 tissue distribution in naturally infected pig-tailed macaques.
| Animal | SHIV1 | Saliva2 | Skin | Cheek | Gingiva | Tonsil | Parotid gland |
Fundic stomach |
Cardiac stomach |
Pyloric stomach |
Spleen | Pancreas | Liver | Kidney |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M03182 | + | − | − | − | − | − | − | 2*103 | 7*103 | 4.9*103 | − | − | − | − |
| K03258 | + | − | − | − | − | − | − | 5.4*104 | − | − | − | − | − | − |
| M04082 | + | − | − | − | − | − | − | 3.1*106 | − | − | − | − | − | − |
| T05183 | − | − | − | − | − | − | 5.7*104 | 9.5*102 | − | 9*103 | − | − | − | − |
| M02156 | − | 2.1*104 | − | 1*104 | − | − | 8.1*101 | − | − | − | − | − | − | − |
| L02393 | − | − | − | − | − | − | 1.1*106 | 2.5*104 | − | − | − | − | − | − |
| M03240 | − | 2.0*105 | − | − | − | − | − | 6.2*106 | − | − | − | − | − | − |
| M05226 | − | 3.6*104 | − | − | − | − | 3.7*104 | 1.9*104 | 7.8*104 | 1*105 | 1*104 | 2.7*104 | 1.9*104 | − |
DNA was isolated from formalin-fixed paraffin-embedded tissue sections obtained at necropsy from five SHIV-positive long-term non-progresssors and seven SHIV-negative macaques that had been differentially vaccinated and challenged with SHIV strain SF162 P4 in a previous SIV vaccine study. Viral loads were determined using the MneHV-6 and cellular OSM qPCR assays in duplicate and are reported as average MneHV-6 genome copies per million cells, as described in Materials and Methods. Two of the SHIV-positive and two of the SHIV-negative animals had undetectable levels of MneHV-6 in all samples tested (not shown).
SHIV status of the animals was determined by weekly testing of SHIV viral loads in plasma by SHIV qPCR
MneHV-6 genome copies / ml whole saliva, which had been collected at various time points at or before necropsy.