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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Biochem J. 2015 Jan 1;465(1):89–101. doi: 10.1042/BJ20140573

Figure 8. Identification of SRC as a direct substrate of PTPδ.

Figure 8

(A) SRC was immunoprecipitated from lysates from MCF10A cells treated either with shPTPRD (for enhanced phosphorylation of Tyr 527) or shMIM (for enhanced phosphorylation of Tyr 416). The immunoprecipitates were treated with recombinant PTPδ, either active wild type (WT) or inactive (CS) mutant proteins, as indicated. Proteins were resolved by SDS-PAGE and immunoblotted using phospho-specific antibodies to Tyr 527 and Tyr 416. (B) MCF10A cells expressing WT and DA mutant were treated with 50 μM pervanadate for 30 minutes. PTPδ was immunoprecipitated, then protein complexes were analyzed by SDS-PAGE and immunoblotted with anti-SRC antibody. (C) Immunoblot analysis of the association of SRC with PTPδ substrate trapping mutant from cell lysates prepared in the absence and presence of vanadate (0.5mM and 1mM).