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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Proteomics. 2014 Aug 27;112:38–52. doi: 10.1016/j.jprot.2014.08.007

Figure 5. NgBR knockdown prevents the TGF-β induced-EMT of MCF-7 breast tumor cells.

Figure 5

(A) NgBR knockdown in MCF-7 cells prevents the cell morphology change from cobblestone-like epithelial appearance to spindle-like mesenchymal type cells after 48 hours of TGF-β treatment at the concentration of 10 ng/ml and 20 ng/ml, respectively. Cell morphology change was determined by images taken with a phase contrast inverted microscope at x 10 magnification. Scale bar = 160 μm. (B) NgBR knockdown decreases TGF-β-induced expression of the mesenchymal marker, vimentin, and TWIST and ZEB1, transcription factors required fro EMT, in MCF-7 breast tumor cells. Protein changes were determined by Western blot analysis.