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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2014 Oct 2;77:20–28. doi: 10.1016/j.yjmcc.2014.09.027

Figure 7. Nur77 inhibits c-Jun dependent transcriptional activity.

Figure 7

(A) HEK-293T cells were transfected with 200 ng c-Jun promoter-luc together with either 200 ng Nur77, DN-Nur77, c-Jun or empty vector as indicated. 36 hours after transfection, luciferase assay was performed. *P<0.05 compared with empty vectors. #P<0.05 compared with c-Jun vector. (B) HEK-293T cells were transfected with c-Jun and Nur77 expression plasmids as indicated for 36 hours and nuclear protein was extracted and EMSA was performed. (C) Quantitative analysis of AP-1 binding intensity from three independent experiments. *P<0.05 compared with either empty vector or Nur77 plus c-Jun. #P<0.05 compared with empty vector.