Table 3.
Spot no.a | GI Accession no.b | Protein name | Protein MW (kDa) | Protein PI | Peptide count | Total ion C.I. %c | Avrerage fold changed | Overall trend* |
---|---|---|---|---|---|---|---|---|
732 | 16741341 | ITIH4 | 104.6 | 6.05 | 4 | 100 | 2.1 ± 0.22 | up |
746 | 16741341 | ITIH4 | 104.6 | 6.05 | 3 | 100 | 1.86 ± 0.24 | up |
1379 | 23956086 | Hemopexin | 51.3 | 7.64 | 3 | 100 | 8.09 ± 2.1 | up |
1410 | 23956086 | Hemopexin | 51.3 | 7.64 | 3 | 100 | 6.95 ± 2.17 | up |
1450 | 54173 | Contrapsin | 46.6 | 5.04 | 8 | 100 | 1.1 ± 0.07 | - |
1798 | 40254615 | SERPINA1 | 45.9 | 5.3 | 2 | 100 | −2.23 ± 0.20 | down |
1991 | 2144486 | Haptoglobin | 38.8 | 6.08 | 2 | 86.7 | 9.79 ±2.5 | up |
2052 | 2144486 | Haptoglobin | 38.8 | 6.08 | 3 | 100 | 67.6 ± 23.7 | up |
2042 | 6754132 | H2-Q10 | 37.3 | 5.13 | 5 | 100 | 19.41 ± 3.9 | up |
2090 | 34785996 | Pregnancy Zone Protein | 165.8 | 6.24 | 3 | 100 | 1.02 ± 0.05 | - |
2102 | 34785996 | Pregnancy Zone Protein | 165.8 | 6.24 | 2 | 100 | 1.1 ± 0.04 | - |
2198 | 37589303 | Collagen, type I, alpha 1 | 117.8 | 5.58 | 2 | 100 | 1.19 ± 0.99 | - |
2242 | 28175786 | Complemnt component 3 | 186.4 | 6.29 | 1 | 100 | 2.32 ± 0.2 | up |
2288 | 38174334 | Serum amyloid P-component | 26.2 | 5.98 | 2 | 100 | 3.04 ± 0.19 | up |
2553 | 56541070 | Transthyretin | 15.8 | 5.77 | 3 | 100 | −1.99 ± 0.098 | down |
Spot no. generatd by DeCycler Image analysis software (v. 7.0, GE Healthcare), referencing the spots shown on the representative image in Figure 3.
Accession number from NCBI database.
C.I.% rates the confidence level of the Protein Score or Ion Score. The closer the Confidence Interval (C.I.%) is to 100%, the more likely the protein is correctly identified.
Average ratio of protein expression between day 12 and day-2, calculated from 3 independent experiments and represented as mean ± SEM.
up: up-regulated in day 12 compared to day-2 group; down: down-regulated in day 12 group comapred to day 2 group; -: no alteration in day 12 compared to day -2.
Abbreviations: SERPINA1: Serpin peptidase inhibitor, clade A, member 1; H2-Q10: Histocompatibility 2, Q region locus 10.