Table 3.

Identification of serum proteins of CAIA model – comparison Day -2 versus Day 12

Spot no.a	GI Accession no.b	Protein name	Protein MW (kDa)	Protein PI	Peptide count	Total ion C.I. %c	Avrerage fold changed	Overall trend*
732	16741341	ITIH4	104.6	6.05	4	100	2.1 ± 0.22	up
746	16741341	ITIH4	104.6	6.05	3	100	1.86 ± 0.24	up
1379	23956086	Hemopexin	51.3	7.64	3	100	8.09 ± 2.1	up
1410	23956086	Hemopexin	51.3	7.64	3	100	6.95 ± 2.17	up
1450	54173	Contrapsin	46.6	5.04	8	100	1.1 ± 0.07	-
1798	40254615	SERPINA1	45.9	5.3	2	100	−2.23 ± 0.20	down
1991	2144486	Haptoglobin	38.8	6.08	2	86.7	9.79 ±2.5	up
2052	2144486	Haptoglobin	38.8	6.08	3	100	67.6 ± 23.7	up
2042	6754132	H2-Q10	37.3	5.13	5	100	19.41 ± 3.9	up
2090	34785996	Pregnancy Zone Protein	165.8	6.24	3	100	1.02 ± 0.05	-
2102	34785996	Pregnancy Zone Protein	165.8	6.24	2	100	1.1 ± 0.04	-
2198	37589303	Collagen, type I, alpha 1	117.8	5.58	2	100	1.19 ± 0.99	-
2242	28175786	Complemnt component 3	186.4	6.29	1	100	2.32 ± 0.2	up
2288	38174334	Serum amyloid P-component	26.2	5.98	2	100	3.04 ± 0.19	up
2553	56541070	Transthyretin	15.8	5.77	3	100	−1.99 ± 0.098	down

^aSpot no. generatd by DeCycler Image analysis software (v. 7.0, GE Healthcare), referencing the spots shown on the representative image in Figure 3.

^bAccession number from NCBI database.

^cC.I.% rates the confidence level of the Protein Score or Ion Score. The closer the Confidence Interval (C.I.%) is to 100%, the more likely the protein is correctly identified.

^dAverage ratio of protein expression between day 12 and day-2, calculated from 3 independent experiments and represented as mean ± SEM.

^*up: up-regulated in day 12 compared to day-2 group; down: down-regulated in day 12 group comapred to day 2 group; -: no alteration in day 12 compared to day -2.

Abbreviations: SERPINA1: Serpin peptidase inhibitor, clade A, member 1; H2-Q10: Histocompatibility 2, Q region locus 10.