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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: J Pharm Sci. 2014 Sep 11;104(2):416–423. doi: 10.1002/jps.24132

Figure 2.

Figure 2

UV-vis spectroscopy confirms Ni(II) is incorporated into the claMP Tag in both Tris-Cl (a) and KPi (b). Ni(II) remains incorporated in the claMP Tag regardless of buffer system chosen, as the intensity of the feature at 310 nm remains constant over the 12-week period with Tris-Cl (c) and KPi (d). At the 24-week time point, the intensity of the feature at 310 nm decreases, suggesting alterations occur in the Ni-claMP complex. The intensity of the feature at 280 nm remains constant, indicating the protein concentration remains the same over the time period investigated.