Fig. 2.

Improved experimental setup to investigate the effect of bulk surface properties on the structure of adsorbed proteins is shown in (a)–(c). The assembled view of the improved setup is shown in panel (a) with the individual components within the setup [one standard spectroscopic grade quartz cuvette (21-Q-10, Starna Cells), six custom-cut fused silica substrates (Custom order CU-1005-041JS, ChemGlass Life sciences), and seven vinyl polymeric spacers] being shown in the exploded view of (b) and (c). The dimensions of each individual component: the standard spectroscopic grade quartz cuvette of path length 1 cm (external dimension: 12.5 mm × 12.5 mm × 45 mm; internal dimension: 42.5 mm × 10 mm × 10 mm), fused silica substrates (9.4 mm × 1.43 mm × 41.2 mm), and T-spacers (base: 1.5 mm × 9.5 mm × 0.2 mm; head: 5.1 mm × 12.5 mm × 0.2 mm) are shown in the front and side views of the setup.