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. 2014 Dec 20;14:3862. doi: 10.1186/s12879-014-0706-x

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© Lu et al.; licensee BioMed Central. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Identifications of SAG5D gene. (A) Constructed plasmid maps and identification of the recombinant plasmids with restriction enzyme digestion. DNA Mark (lane M), pEGFP-C1 digested with BamH I (lane 1), pEGFP-C1-SAG5D digested with BamH I (lane 2), pEGFP-C1-SAG5D digested with Kpn I and BamH I (lane 3). (B) In vivo expression analysis of the constructs in mice by RT-PCR. DNA Mark (lane M), PBS (lane 1), α-GalCer (lane 2), pEGFP-C1 (lane 3), pEGFP-C1-SAG5D (lane 4), pEGFP-C1-SAG5D/α-GalCer (lane 5).